Reads in a pdb and emails the user with predicted hydration shell densities and crystallographic water sites. To learn more about this click here.

Enter PDB file. HyPred may not be able to handle proteins with more than 1000 residues well.

Add hydrogen atoms? Learn more about this here.

Yes
No

Eliminate waters in cavities? Learn more about this here.

Yes
No

Density cut off (e-/Å^3, uniform water is 0.33 e-/Å^3). Points with a density greater than this value (and with no points with a greater density within a 1.4 Å radius) are the predicted high occupancy (e.g crystallographic) waters. Values between 1-4 e-/Å^3 are suggested.

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Please cite Virtanen, Jouko, Makowski, Lee, Sosnick, Tobin R., & Freed, Karl F. (2010) Modeling the hydration layer around proteins: HyPred Biophysical Journal 99:1611-1619 if you are using this web server.